Modified Primers – 5′-Cal flour Red 610



PentaBase manufactures standard and custom DNA primers. PentaBase has furtermore developed a primer technology completely compatible with all applications of your standard primers. The primers are based on standard DNA nucleotides, added one or a few pentabase modifications.


SuPrimers‚ are DNA primers modified with the proprietary pentabases and compatible with all applications of your standard primers. SuPrimers‚ can be used with all your standard reagents. Adding pentabases to your primer will improve one or more of the following features:

Affinity ‚ ì Adding a pentabase to an oligo nucleotide increases affinity towards DNA. This means that your primer can work at higher temperatures

Sensitivity ‚ ì pentabases increase the on-target portion of an oligo and thereby the sensitivity of the primer

Primer-dimer. Adding a pentabase to or near the 5‚ ≤-end of an oligo, will increase the interaction of the 5‚ ≤ ends of unbound primers. This reduces the tendency to 3‚ ≤-end interactions needed to make primer-dimer. Hence, the result is reduced primer-dimer formation

  • Specificity. A SuPrimer can be made shorter than a standard primer and thereby be more specific in it‚ binding to a target sequence. However, please note that the primer sequence should still need to be unique in order not to mis-prime.
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  • Store Name: Pentabase
  • Vendor: Pentabase
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